Fluorescence in situ hybridisation detection of erbB2 amplification in breast cancer fine needle aspirates
نویسندگان
چکیده
منابع مشابه
HER-2/neu amplification detected by fluorescence in situ hybridization in fine needle aspirates from primary breast cancer.
BACKGROUND The HER-2/neu gene is amplified in 20-30% of human breast cancers and has been shown to have prognostic and predictive value for treatment with chemotherapy, hormone therapy and antibodies against the HER-2/neu domain (trastuzumab). The aim of our study was to evaluate the reliability of HER-2/neu determination by fluorescence in situ hybridization (FISH) on fine-needle aspirates (FN...
متن کاملRapid fluorescence in situ hybridisation (FISH) for HER2 (ERBB2) assessment in breast and gastro-oesophageal cancer.
Evaluation of HER2 (ERBB2) gene amplification or protein expression is standard of care in breast (BR) and advanced stage gastro-oesophageal cancers to identify patients eligible for anti-HER2 therapies. Here, we evaluate a rapid fluorescence in situ hybridisation (FISH) technology (HER2 instant quality (IQ) FISH pharmDx Kit) for detection of HER2 in patients with BR and gastro-oesophageal canc...
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The goal of this work is to determine the accuracy of computer-based image analysis in diagnosing breast fine-needle aspirates (FNA). On 192 FNAs, the computerbased diagnostic accuracy was 97.9%. This is consistent with the 97.5% accuracy projected by machine learning methods during the initial training in 1994 with 569 FNAs. One of the attributes of this system is the rendering of a value that...
متن کاملLiquid-based fluorescence in situ hybridization assay for detection of ERBB2 gene amplification in patients with breast cancer.
BACKGROUND Current reference methods for evaluating gene amplification and expression of ERBB2 (also known as HER-2)--cell-based fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC)--are subjective and influenced by methods of tissue preparation and fixation. We developed and evaluated a novel, quantitative liquid-based FISH (L-FISH) assay that uses flow cytometry to detect ...
متن کاملDetection of mitochondrial DNA mutations in primary breast cancer and fine-needle aspirates.
To determine the frequency and distribution of mitochondrial DNA mutations in breast cancer, 18 primary breast tumors were analyzed by direct sequencing. Twelve somatic mutations not present in matched lymphocytes and normal breast tissues were detected in 11 of the tumors screened (61%). Of these mutations, five (42%) were deletions or insertions in a homopolymeric C-stretch between nucleotide...
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ژورنال
عنوان ژورنال: Molecular Pathology
سال: 1999
ISSN: 1366-8714
DOI: 10.1136/mp.52.2.75